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Objective@#The proposal of this study is to determine the feasibility of using online social software to guide popular opinion leaders(POLs) among young students MSM (men who have sex with men, MSM) carrying out comprehensive AIDS prevention in young students MSM.@*Methods@#From November to December 2019 in Tianjin, 22 POLs who had previously experienced AIDS prevention education and training foucus on life skills were recruited. Though snowball sampling method, 100 POLs partners (friends, sexual partners, classmates, etc) were invited into the study. From January to March 2020, regular posters will be sent through the WeChat platform to guide POL to intervene their partners/sexual partners. The questionnaire surveys were conducted before and after the intervention.@*Results@#After the intervention, both the proportion of young students MSM had sex and with multiple sex partners decreased(χ2=12.12, 5.94, P<0.05). The comprehensive ability of AIDS prevention has improved significantly, the "National Eight Items" correct rate has risen from 90.0% to 99.0%(χ2=5.82, 7.56, P<0.05), and the "Youth Eight Items" correct rate has increased from 86.0% to 98.0%(P<0.01). Knowledge score increased from(11.08±1.89) to (11.84±1.27); Attitude score increased from (54.29±4.01) to (56.06±3.75), the behavior score increased from (52.12±4.78) to (55.08±4.25), skill score increased from (51.96±4.75) to (54.67±5.04), The comprehensive prevention ability score (percent) increased from (87.30±6.95) to (91.86±5.42)(t=-4.21, -3.51, -5.32, -5.16, -6.45, P<0.01).@*Conclusion@#Using Wechat platform to guide POL carrying out comprehensive AIDS prevention is effective for improving the comprehensive AIDS prevention ability of young students MSM.
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Objective This study was to study the effect of monovision contact lens (MV-CL) on distant and near vision,distant and near stereopsis,and to identify the effect of degree of monovision on vision function.Methods A prospective case observational study was designed.The clinical data of sixty-eight eyes of 47 cases were collected from November 2010 to August 2014 in Henan Provincial Corps Hospital,Chinese People's Armed Police Forces.Sixty-eight eyes of 47 cases wore contact lenses to improve near vision,in which the other eye of 21 cases with hyperopia wearing contact lenses to improve distant vision.Besides,far and near stereopsis were measured before and two weeks after wearing lenses respectively;and scotopia were measrued with lenses and in naked eyes respectively.Compared the changes of vision and stereopsis in patients before and after the MV-CL,and conducted the patient satisfaction survey.Results There were 89％ (42/47 cases) and 6％ (3/47 cases) of the patients whose naked far and near vision was 0.8/0.33 (J4) at 2 weeks after MV-CL and before MV-CL,respectively.There were no statistical significance about change of near stereopsis with Titmus stereopicture and the Yan's near random-dot stereogram (x2=1.30,0.56;both at P＞0.05).There were no statistical significance about change with apparent machine random dot stereogram iterative crossed parallax and uncrossed disparity (x2 =0.16,0.11;both at P＞0.05).The far distance fusion range and far stereopsis were not significantly different between before and after MV-CL (x2 =0.22,0.16;both at P＞0.05).Titmus stereopicture tests showed that there was a decrease in the number of people who had foveal stereopsis,while an increase was found in people who had macular stereopsis after MV-CL,but the difference had no statistical significance (x2 =2.28,P ＞ 0.05).Conclusions Near vision can be obviously increased and presbytism can be relieved by MV-CL.Stereopsis of some patients are decreased after MV-CL,but this can be accepted by the patients and there is no obvious decrease in far range of fusion.
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To investigate the relationship between the expression of death associated protein kinase (DAPK) gene and oral squamous cell carcinoma (OSCC). The expression of DAPK gene in OSCC was detected by semi-quantitive RT-PCR. The results showed that the expression of DAPK gene was down regulated significantly, which may relate to the tumorigenesis and development of OSCC. The detection of DAPK gene may act as an index in OSCC dignosis.
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Objective To study the expression of fragile histidine triad(FHIT) in oral squamous cell carcinoma(OSCC),and discuss its role and significance in OSCC.Methods Immunohistochemical method was used to detect the expressions of FHIT in 48 cases of OSCC and 26 normal oral mucosa.Results The positive expression rate of FHIT in normal oral mucosa was 76.92%(20/26).Among 48 OSCC patients,the positive rate of FHIT(43.75%) was lower than that in normal oral mucosa(P0.05),but FHIT protein content was significantly associated with differentiation(P
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Objective To investigate the expression of c-jun in precancerous lesion and squamous cell carcinoma tissues of hamster buckle pouch mucosa.Methods The models of premalignant and malignant lesions were established in hamster buckle pouch mucosa induced with DMBA.The expressions of c-jun in 23 oral normal mucosa, 22 abnormal epithelial hyperplasia and 29 oral squamous cell carcinoma (OSCC) were measured with immunohistochemical SABC method.Results c-jun mainly expressed in basal cells and prickle cells in normal mucosa,simple and abnormal epithelial hyperplasia,its positive expression was in nucleus.c-jun expressed mainly in various layer cells of OSCC,its positive expression was in nucleus and cytoplasm.There were significant differences of positive expression rate between various groups(?2=13.71,P
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Objective To clone the human bone morphogenetic protein-2(hBMP2) gene cDNA and construct its replication-deficient type 5 adenoviral vector.Methods Using osteoblastic sarcoma tissue to extract cell total RNA,hBMP-2 cDNA was amplified by RT-PCR.The hBMP-2 cDNA was then inserted into cloning vector pGEM-T.The recombinant plasmid was screened and the recombinant plasmid which was confirmed by restriction enzyme,PCR and sequence analysis was gotten.To construct the recombinant adenoviral vector,the BMP-2 cDNA was subcloned into a shuttle vector and the recombinant pShuttle-BMP2 vector was obtained.It was subcloned into Adeno-X adenoviral genomic DNA and the pAd-BMP2 DNA was obtained.Results A length of 1 200 bp BMP-2 cDNA was cloned,and it was subcloned into adenoviral vector,the recombinant adenovirus was constructed successfully.After measuring the titre of virus(1?1011 nfu?L-1),the target gene was evaluated by PCR.Conclusion The hBMP-2 adenoviral is obtained.The successful construction of pAd-BMP2 may provide us useful target for the further research.
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Objective:To explore the relationship between apoptotic protease activating factor 1(APAF1) gene and oral squamous cell carcinoma (OSCC). Methods:The mRNA expression of APAF1 gene were detected with semiquantitative RT-PCR method in 18 cases of normal oral mucous membrane and 32 cases of OSCC tissues. Results:The expression of APAF1 gene was significantly decreased in OSCC tissues comparing with the normal oral mucous membrane(P
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Objective:To elucidate the role of dpc4 gene in the development of squamous cell carcinoma. Methods:DMBA at 5 g/L was locally applied in check pouch 3 times a week for 6,9 and 12 weeks respectively in three groups of golden hamasters to induce carcinoma.The animals were sacrificed after DMBA application.Normal controls were the check pouch samples of golden hamasters without any treatment and negative controls were established by local application of acetone for 12 weeks.DPC4 4 expression was examined by immunohistochemical staining.Results:DPC4 expression was observed in all(22) cases of the normal epithelium samples(100%),20 of the 21 cases of simple hyperplasia(95.2%),20 of the 27 cases of abnormal hyperplasia(74.1%) and 12 of the 25 cases of squomous cell carcinoma(48%).Conclusion:dpc4 may play an important role in the development of oral squomous cell carcinoma.
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Objectives:To study the role of COX-2 and bFGF in the development of oral squamous cell carcinoma(OSCC).Methods:The expressions of COX-2 and bFGF were exmined by immunohistochemical staining with anti-COX-2 and anti-bFGF monoclonal antibodies respectively in 23 cases of OSCC,25 of oral leukoplakia(OLK)and 10 of normal oral mucosa.Results:COX-2 was detected in 20/23 cases of OSCC,13/25 of OLK and 0/10 of normal oral mucosa(P
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Objective: To observe the expression of proteins Rb and P 53 in leukoplakia (LK) and squamous cell carcinoma (SCC) of oral muco sa. Methods: The expression of proteins Rb and P 53 was determined by immunohistochemical S-P method. Paraffin sections of 33 s amples of SCC and 20 of LK were immunohistochemically stained and analyzed. Results: (1)The expression of Rb in OLK, OSCC was not si gnificantly different from that in normal oral mucosa ;(2)the overexpression of mutant P 53 were found in OLK and its expression was increasing with dysp hasia degree (P
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Objective:To develop bifunctional anti-human lingual cancer/anti-VEGF antibody.Methods:Antigen of lingual cancer was prepared from Tca 8113 cells and that of VEGF from endothelial cells of umbilical cord vein.The hybridoma cells of anti-human ligual cancer/anti-VEGF were prepared by the technique of secondary hybridoma.The antibody was identified by ELISA. Results:The specific conjugation rate between bifunctional antibody and lingual cancer tissue was 85.29%, and that between the antibody and vascular endothelial cells was 82.35%.Conclusion:The bifunctional antibody of anti-human lingual cancer/anti-VEGF has good targeting potential.
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Objective:To investigate ?-catenin expression during check pouch carcinogenesis in golden hamster.Methods:Expression of ?-catenin was examined with immunohistochemical SABC method in 23 cases of check normal buccal epithelium of golden hamster,22 of hyperplasitic epithelium,22 of dysplastic epithelium,29 of squamous cell carcinoma.Results:Normal buccal epithelium showed strong pericellular staining in the basal,suprabasal and prickle cell layers.Positive rates(%) of ?-catenin in normal buccal epithelium,hyperplastic epithelium,dysplastic epithelium and squamous cell carcinoma were 100,100,77.3 and 41.4 respectively.Conclusion: The decreased expression of ?-catenin may be related to the carcinogenesis of golden hamster check pouch.